Although WNT signaling plays a prominent role in A

2018-10-20

Although WNT signaling plays a prominent role in A/P patterning of the neural tube, few studies have extensively examined the influence of WNT on the A/P positional identity of hPSC-derived NPCs and neurons. To date, most studies have relied on FGF8 (Yan et al., 2005; Yang et al., 2008) or retinoic

kainate receptors Main Text br Acknowledgments The

2018-10-20

Main Text Acknowledgments The authors would like to thank all delegates for their input into the workshops that led to the contents of this white paper (Table S2). Introduction The type II clustered regularly interspaced short palindromic repeat (CRISPR) systems and the associated Cas9 nucle

Direct cell reprogramming technology is

2018-10-20

Direct cell-reprogramming technology is based on the dominant action of cell-lineage transcription factors (TFs) in converting adult somatic cells into different cell types (Graf and Enver, 2009). This technique represents a promising avenue in the field of regenerative medicine, with the potential

br Experimental Procedures br Acknowledgments

2018-10-20

Experimental Procedures Acknowledgments Introduction Spermatogonial stem Retigabine dihydrochloride (SSCs) give rise to the spermatogenesis that endures throughout the life of male animals (de Rooij and Russell, 2000; Meistrich and van Beek, 1993). SSCs reside in a special microenvironment

One difficulty in deriving EpiSCs is the need for

2018-10-20

One difficulty in deriving EpiSCs is the need for microdissection of small postimplantation embryos. The original protocols used only the epiblast layer separated from the surrounding visceral endoderm (VE) tissue as a source of the cell lines. The overall efficiency of EpiSC derivation from epiblas

The following are the supplementary data related

2018-10-20

The following are the supplementary data related to this article. Conflict of interest Introduction Mammalian eyes contain retinal stem cells (RSCs) that are capable of proliferating and differentiating into all of the cell types of the neural retina as well as the retinal pigmented epitheliu

In this study lentiviral vectors were used to ablate

2018-10-20

In this study, lentiviral vectors were used to ablate CXCR4 and/or CXCR7 expression using shRNAs, and the derived hiPSC-CMs were tested for phenotypic and functional properties due to gene knockdown. Gene expression and flow cytometry studies confirmed the cardiomyocyte phenotype of the differentiat

TAU fragments carry an apoptosis

2018-10-20

TAU fragments carry an apoptosis-inducing activity, which is further potentiated when mdm2 inhibitor are exposed to TAU fragments carrying the N279K mutation (Fasulo et al., 2005). In addition, overexpression of full-length TAU carrying the N279K or the V337M mutation resulted in induction of apopto

We previously discovered that ATM

2018-10-20

We previously discovered that ATM, in addition to serving many roles in cell-cycle regulation and DNA damage response, also functions as a key epigenetic regulator in neurons, likely underlying the ataxic symptoms as well as neurodegeneration. ATM indirectly regulates nuclear-cytoplasmic shuttling o

Cardiac MRI was performed to

2018-10-20

Cardiac MRI was performed to assess potential changes in cardiac function due to teratoma growth. For this, a subacute model with cell delivery 4 days after ischemia reperfusion injury was chosen since functional improvements following the transplantation of human ESC-CMs have been reported for this

br Experimental Procedures br Author Contributions br Introd

2018-10-20

Experimental Procedures Author Contributions Introduction Bone-marrow-derived mesenchymal stromal beta amyloid (BM-MSCs) have been reported to secrete neurotrophic cytokines as well as a range of pro-inflammatory cytokines, chemokines, and other soluble growth factors (Nakano et al., 2010;

Aside from the benefits of the actual protocol

2018-10-20

Aside from the benefits of the actual protocol, our method also results in hiNSCs with multiple favorable properties including a seemingly endless capacity for self-renewal. hiNSC colonies grown on MEF feeders are approximately 50% positive for proliferation marker Ki67, a characteristic that does n

We have confirmed that established iPS RPE

2018-10-20

We have confirmed that established iPS-RPE cells constitutively express MHC-I but not MHC-II. However, the cytokines produced acutely in response to immune rejection may upregulate RPE MHC-II expression, as seen in vitro. This upregulation may be sufficient to initiate a recognition response in vivo

Here we attempted to reprogram highly fluorescence activated

2018-10-20

Here, we attempted to reprogram highly fluorescence-activated cell sorting (FACS)-purified (100% purity) leukemia blasts from three subtypes of B-ALL, t(4;11)/MLL-AF4+, t(1;11)+MLL-EPS15+, and t(12;21)/ETV6-RUNX1 B-ALL, to establish novel iPSC-based disease models to address the developmental impact

Hello world!

2018-07-29